05 Bacterial Genetics

Growth

  • put bacteria in flask, watch growth
  • initial no bacteria, then exponential growth, plateau, loss

  • no growth. May be growing in size but not dividing

  • exponential, log
  • antibiotics greatest effect

  • dying from not enough nutrients
  • death = growth

Gene Transfer

Transformation

  • DNA in environment or lab experiment
  • micro labs: genes in culture media for replication

Conjugation

  • DNAase degrade DNA freely in culture

  • incorporate plasmids in DNA
  • can transfer entire genome or partial genome to another bacteria without plasmid

  • plasmid transferred and drags portion of bacteria with it

Transduction

Generalized

  • DNA potentially incorporated

Specialized

  • viral DNA live in bacterial genome for a while

Lytic

  • lytic: generalized transduction, lyses bacteria infected
  • lysogenic: may later become lytic or just live inside bacteria

Lysogenic

Transposition

  • jumping genes